譜析(上海)生物科技有限公司優(yōu)勢供應:T4 RNA Ligase 2(T4 RNA連接酶2)
2019/10/12 14:34:21
T4 RNA連接酶催化RNA的5個磷酸和3個羥基之間形成磷酸二酯鍵。它最活躍的是密封缺口的雙鏈RNA,但它也會加入單鏈RNA的末端,可以連接RNA的3個羥基與DNA的5個磷酸形成雙鏈形式。酶需要ATP,除非底物的5端已預先腺苷化。
T4 RNA ligase catalyses the formation of a phosphodiester bond between a 5′ phosphate and 3′ hydroxyl of RNA. It is most active in sealing nicked double-stranded RNA but it will also join the ends of single-stranded RNA and can ligate the 3′ hydroxyl of RNA to the 5′ phosphate of DNA in a double stranded form. The enzyme requires ATP unless the 5’ end of the substrate is preadenylated.
優(yōu)勢供應產(chǎn)品:
Cat No. | Product |
TRL201 | Kit 1 2,000U T4 RNA ligase 2 (20U/uL; 100uL), 10x buffer |
TRL202 | Kit 2 10,000U T4 RNA ligase 2 (20U/uL; 500uL), 10X buffer |