譜析（上海）生物科技有限公司優(yōu)勢(shì)供應(yīng)：T4 RNA Ligase 1(T4 RNA連接酶1)
2019/10/12 14:53:05
T4 RNA連接酶1催化在單鏈RNA或DNA的末端5個(gè)磷酸和3個(gè)羥基之間形成磷酸二酯鍵。它需要ATP作為輔助因子。一個(gè)單位被定義為將50%等摩爾(125nM)的單鏈5 fam標(biāo)記的17-mer RNA連接到5個(gè)磷酸化的18-mer RNA上所需要的酶量，20 L, 37 C, 30分鐘。
T4 RNA Ligase 1 catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of single-stranded RNA or DNA. It requires ATP as a cofactor.
One unit is defined as the amount of enzyme required to ligate 50% of an equimolar mix (125nM) of a single-stranded 5′ FAM-labeled 17-mer RNA to a 5′ phosphorylated 18-mer RNA in 20 μL at 37°C for 30 minutes.
優(yōu)勢(shì)供應(yīng)產(chǎn)品：
| Cat No. | Product |
| TRL101 | Kit 1 2,000U T4 RNA ligase 1 (20U/uL; 100uL), 10X buffer |
| TRL102 | Kit 2 10,000U T4 RNA ligase 1 (20U/uL; 500uL), 10X buffer |
