譜析（上海）生物科技有限公司優(yōu)勢供應(yīng)：T4 DNA Ligase
2019/10/12 14:57:07
Inspiralis提供T4 DNA連接酶已有多年。我們可以少量供應(yīng)，也可以大量供應(yīng)，或者更集中供應(yīng)(例如，用于dna標(biāo)記化學(xué)圖書館建設(shè))。T4 DNA連接酶催化磷酸二酯鍵在末端5磷酸和3個雙鏈DNA或RNA羥基之間形成。它可以連接DNA或寡核苷酸的粘性末端，以及RNA和RNA-DNA的雜交體，在雙鏈DNA上尤其有效。它也可以連接鈍端DNA，但不能連接單鏈核酸。它需要ATP作為輔助因子。T4 DNA連接酶以2000 u /ul或13000 u /ul的速度供應(yīng)，并提供10X緩沖。1U是一個內(nèi)聚的末端連接單元(CELU)。650個CELUs大約是一個Weiss單元，所以2000個CELUs/ul相當(dāng)于~3個Weiss單元/ul (TDL1, TDL2)或20個Weiss單元/ul (TDL3C)
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA. It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)
優(yōu)勢供應(yīng)產(chǎn)品：
譜析（上海）生物科技有限公司是一家致力于為實驗室分析領(lǐng)域
更多產(chǎn)品請咨詢上海熹垣生物。
Inspiralis has been supplying T4 DNA ligase for many years. We can supply it in the smaller quantities below or in larger or more concentrated quantities (for example for DNA-tagged chemical library construction).
T4 DNA ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. It can ligate cohesive ends of DNA or oligonucleotides, as well as RNA and RNA-DNA hybrids and is particularly efficient at sealing nicks in duplex DNA. It can also ligate blunt-ended DNA but cannot ligate single-stranded nucleic acids. It requires ATP as a cofactor.
T4 DNA ligase is supplied at either 2,000U/ul or 13,000U/ul along with 10X buffer. 1U is a cohesive end joining unit (CELU). 650 CELUs is approximately 1 Weiss unit so 2000 CELUs/ul is equivalent to ~3 Weiss units/ul (TDL1, TDL2) or 20 Weiss units/ul (TDL3C)
優(yōu)勢供應(yīng)產(chǎn)品：
| CatNo. | Product |
| TDL1 | Kit 1 80,000U T4 DNA ligase (2000U/ul), 10X buffer |
| TDL2 | Kit 2 400,000U T4 DNA ligase (2000U/ul), 10X buffer |
| TDL3C | Kit3 400,000U T4 DNA ligase (13,000U/ul), 10X buffer |
