譜析(上海)生物科技有限公司優(yōu)勢供應:S.pneumoniae Gyrase(肺炎促旋酶)
2019/10/12 15:38:45
肺炎鏈球菌回轉(zhuǎn)酶是一種能將負超螺旋引入DNA的II型拓撲異構(gòu)酶。它是喹諾酮類和香豆素類藥物的靶點,可用于篩選潛在的抗菌化合物。其制備方法是在大腸桿菌中過表達亞基,然后進行純化。在稀釋緩沖液中以A2B2絡合物的形式提供。儲存于-80℃。所有酶都提供5X濃縮的分析緩沖液和稀釋緩沖液,也可單獨使用。1 U的gyrase將在37℃條件下30分鐘內(nèi)超螺旋化0.5 g的pBR322 DNA。
Streptococcus pneumoniae gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 μg relaxed pBR322 DNA in 30 minutes at 37 °C.
優(yōu)勢供應產(chǎn)品:
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Streptococcus pneumoniae gyrase is a bacterial type II topoisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared by overexpressing the subunits in E. coli and then purified.
It is supplied as an A2B2 complex in Dilution Buffer. Store at -80 °C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 μg relaxed pBR322 DNA in 30 minutes at 37 °C.
優(yōu)勢供應產(chǎn)品:
CatNo. | Product | Product description |
SPG1001 | S. pneumoniae Gyrase |
100U |
SPG1002 | S. pneumoniae Gyrase |
500U |