譜析(上海)生物科技有限公司優(yōu)勢(shì)供應(yīng):M.tuberculosis Topo I
結(jié)核分枝桿菌拓?fù)洚悩?gòu)酶I是一種能夠放松超螺旋DNA的I型拓?fù)洚悩?gòu)酶。它是一個(gè)潛在的抗菌藥物靶點(diǎn),可用于篩選潛在的新型抗菌化合物。該蛋白在大腸桿菌中過表達(dá)后經(jīng)色譜純化得到。它是用稀釋緩沖液供應(yīng)的。儲(chǔ)存于-80℃,酶液中分別提供5倍濃度的緩沖液和1倍稀釋緩沖液。1 U of topo I將在37℃下30分鐘內(nèi)放松0.5 g超螺旋pBR322 DNA。
Mycobacterium tuberculosis topoisomerase I is a bacterial type I topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by chromatography.
It is supplied in Dilution Buffer. Store at -80 °C.
The enzyme is supplied with Assay Buffer (5X concentration) and Dilution Buffer (1X) which are also available separately. 1 U of topo I will relax 0.5 μg of supercoiled pBR322 DNA in 30 minutes at 37 °C.
優(yōu)勢(shì)供應(yīng)產(chǎn)品:
CatNo. | Product | Product description |
MTT01 | M.tuberculosis Topo I |
100U |
MTT02 | M.tuberculosis Topo I |
500U |