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2019/10/14 11:42:06
Topo IV(來自肺炎鏈球菌)是通過在大腸桿菌中過量表達亞基并通過內(nèi)部開發(fā)的方法純化它們而制備的。亞基經(jīng)SDS-PAGE純化至>95%純度。topo IV在稀釋緩沖液中作為異四聚體復(fù)合物提供。建議重新引用酶,以避免重復(fù)的凍融循環(huán)。儲存于-80℃。所有酶都提供5X濃縮的分析緩沖液和稀釋緩沖液,也可單獨使用。
Topo IV (from Streptococcus pneumoniae) is prepared by overexpressing the subunits in E. coli and purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex  in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80oC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
優(yōu)勢供應(yīng)產(chǎn)品:
 
CatNo. Product Product description
SPT4001 S. pneumoniae Topo IV
 
100U
SPT4002 S. pneumoniae Topo IV
 
500U

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