譜析（上海）生物科技有限公司提供——Vitamin B1, B2 in Whole Blood and B6 in Plasma / Whole Blood (Combikit) 質(zhì)譜診斷方案
2022/3/4 14:32:02
譜析（上海）生物科技有限公司提供——Vitamin B1, B2 in Whole Blood and B6 in Plasma / Whole Blood (Combikit) 質(zhì)譜診斷方案
一、說明
The combi kit enables the combined measurement of the vitamins B1, B2, and B6. It consists of the chromatography kit (order no. 27000) and appropriate sample preparation kits (ordernos. 27001, 27002, 27006). The chromatography is performed with a single column for all B-vitamins in subsequent series of measurement. For switching the parameter (e.g. vitamin B1 --> vitamin B2) the parameter settings have to be changed (see HPLC parameters). By selecting the appropriate sample preparation kits, the customer is flexible to choose the parameters ccording to his needs.
二、產(chǎn)品優(yōu)勢(shì)
（1）High flexibility:
1 Chromatography kit
+ 3 sample preparation kits
三、測試數(shù)據(jù)
Test data are available on request.
四、高效液相色譜參數(shù) 維生素B1
Pump (Mobile Phase A + B): flow rate 1 ml/min ratio A: B = 93: 7
Pump (Reagent N): flow rate 0.1 ml/min
Injection volume: 50 μI
Injection interval: 6 min
HPLC-Thermostat: 35°C
Retention times:
TPP approx. 2.5 min
五、高效液相色譜參數(shù) 維生素B2
Pump (Mobile Phase A + B): flow rate 1 ml/min ratio A: B = 80: 20
Injection volume: 50 μI
Injection interval: 10 min
HPLC-Thermostat: 40°C
Detector (exc. / em.): 450 nm / 530 nm
Retention times:
FAD approx. 2.8 min
六、高效液相色譜參數(shù) 維生素B6
Pump (Mobile Phase A + B): flow rate 1 ml/min ratio A: B = 100: 0
Pump (Reagent N): flow rate 0.2 ml/min
Injection volume: 50 μI
Injection interval: 10 min
HPLC-Thermostat: 25°C
Detector (exc. / em.): 370 nm / 470 nm
Retention times:
PLP approx. 5.2 min
七、樣品制備
樣品制備維生素B1見第58頁（第二十四個(gè)），維生素B2見第60頁（第二十五個(gè)），維生素B6見第62頁（第二十六個(gè)）
八、色譜圖
參見第59頁(維生素B1，第二十四個(gè))、61頁(維生素B2，第二十五個(gè))和63頁(維生素B6，第二十六個(gè))的色譜圖
九、訂購信息
The combi kit consists of the chromatography kit (order no. 27000) and the separate sample preparation kits (order nos. 27001, 27002 and 27006).
十、色譜法
十一、樣品制備
更多產(chǎn)品請(qǐng)咨詢譜析（上海）生物科技有限公司。
網(wǎng)址： haoxd.cn
電話：021-58116080
手機(jī)：17717017426 陳經(jīng)理 18019263206 陸經(jīng)理
譜析（上海）生物科技有限公司，您實(shí)驗(yàn)室試劑供應(yīng)專家，歡迎咨詢?cè)斦劇?br>
一、說明
The combi kit enables the combined measurement of the vitamins B1, B2, and B6. It consists of the chromatography kit (order no. 27000) and appropriate sample preparation kits (ordernos. 27001, 27002, 27006). The chromatography is performed with a single column for all B-vitamins in subsequent series of measurement. For switching the parameter (e.g. vitamin B1 --> vitamin B2) the parameter settings have to be changed (see HPLC parameters). By selecting the appropriate sample preparation kits, the customer is flexible to choose the parameters ccording to his needs.
二、產(chǎn)品優(yōu)勢(shì)
（1）High flexibility:
1 Chromatography kit
+ 3 sample preparation kits
三、測試數(shù)據(jù)
Test data are available on request.
四、高效液相色譜參數(shù) 維生素B1
Pump (Mobile Phase A + B): flow rate 1 ml/min ratio A: B = 93: 7
Pump (Reagent N): flow rate 0.1 ml/min
Injection volume: 50 μI
Injection interval: 6 min
HPLC-Thermostat: 35°C
Retention times:
TPP approx. 2.5 min
五、高效液相色譜參數(shù) 維生素B2
Pump (Mobile Phase A + B): flow rate 1 ml/min ratio A: B = 80: 20
Injection volume: 50 μI
Injection interval: 10 min
HPLC-Thermostat: 40°C
Detector (exc. / em.): 450 nm / 530 nm
Retention times:
FAD approx. 2.8 min
六、高效液相色譜參數(shù) 維生素B6
Pump (Mobile Phase A + B): flow rate 1 ml/min ratio A: B = 100: 0
Pump (Reagent N): flow rate 0.2 ml/min
Injection volume: 50 μI
Injection interval: 10 min
HPLC-Thermostat: 25°C
Detector (exc. / em.): 370 nm / 470 nm
Retention times:
PLP approx. 5.2 min
七、樣品制備
樣品制備維生素B1見第58頁（第二十四個(gè)），維生素B2見第60頁（第二十五個(gè)），維生素B6見第62頁（第二十六個(gè)）
八、色譜圖
參見第59頁(維生素B1，第二十四個(gè))、61頁(維生素B2，第二十五個(gè))和63頁(維生素B6，第二十六個(gè))的色譜圖
九、訂購信息
The combi kit consists of the chromatography kit (order no. 27000) and the separate sample preparation kits (order nos. 27001, 27002 and 27006).
十、色譜法
十一、樣品制備
更多產(chǎn)品請(qǐng)咨詢譜析（上海）生物科技有限公司。
網(wǎng)址： haoxd.cn
電話：021-58116080
手機(jī)：17717017426 陳經(jīng)理 18019263206 陸經(jīng)理
譜析（上海）生物科技有限公司，您實(shí)驗(yàn)室試劑供應(yīng)專家，歡迎咨詢?cè)斦劇?br>
