譜析(上海)生物科技有限公司優(yōu)勢供應(yīng):E。桿菌促旋酶 (E.coli Gyrase)
DNA螺旋酶是一種能將負(fù)超螺旋引入DNA的細(xì)菌型拓?fù)洚悩?gòu)酶。它是喹諾酮類和香豆素類藥物的靶點(diǎn),可用于篩選潛在的抗菌化合物。以高產(chǎn)菌株JMtacA和JMtacB1為原料,以A2B2絡(luò)合物的形式提供。儲(chǔ)存于-80℃。所有酶都提供5X濃縮的分析緩沖液和稀釋緩沖液,也可單獨(dú)使用。1 U的gyrase將在30分鐘內(nèi)超螺旋化0.5 g的pBR322 DNA (37 c)。
DNA gyrase is a bacterial type II topisomerase which can introduce negative supercoils into DNA. It is a target for both quinolone and coumarin drugs and can be used for screening potential antibacterial compounds. It is prepared from the over producing strains JMtacA and JMtacB1 and is supplied as an A2B2 complex.
Store at -80°C.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution Buffer which are also available separately. 1 U of gyrase will supercoil 0.5 μg relaxed pBR322 DNA in 30 minutes at 37°C.
優(yōu)勢供應(yīng)產(chǎn)品:
Cat No. | Product | Size | Quantity |
G1001 | E.coli gyrase |
100 U | |
G5002 | E.coli gyrase |
500 U | |
G1003 | E.coli gyrase |
1000 U | |
G2004 | E.coli gyrase |
2000 U |
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