譜析(上海)生物科技有限公司優(yōu)勢(shì)供應(yīng):S.aureus Topoisomerase IV(金黃色葡萄球菌拓?fù)洚悩?gòu)酶IV)
2019/10/12 14:14:51
Topo IV(來(lái)自金黃色葡萄球菌)是通過(guò)在大腸桿菌中過(guò)量表達(dá)亞基并通過(guò)內(nèi)部開(kāi)發(fā)的方法純化它們來(lái)制備的。亞基經(jīng)SDS-PAGE純化至>95%純度。topo IV在稀釋緩沖液中作為異四聚體復(fù)合物提供。建議重新引用酶,以避免重復(fù)的凍融循環(huán)。儲(chǔ)存于-80℃。所有酶都提供5X濃縮的分析緩沖液和稀釋緩沖液,也可單獨(dú)使用。
Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coliand purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80oC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
優(yōu)勢(shì)供應(yīng)產(chǎn)品:
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更多產(chǎn)品請(qǐng)咨詢(xún)上海熹垣生物。
Topo IV (from Staphylococcus aureus) is prepared by overexpressing the subunits in E. coliand purifying them by methods developed in-house.
The subunits are purified to >95% purity as judged by SDS-PAGE. The topo IV is supplied as a heterotetramer complex in Dilution buffer.
It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles. Store at -80oC.
All enzyme is supplied with 5X concentrated Assay Buffer and Dilution buffers which are also available separately.
優(yōu)勢(shì)供應(yīng)產(chǎn)品:
CatNo. | Product | Product description |
SAT4001 | S. aureus Topo IV |
100 U |
SAT4002 | S. aureus Topo IV |
500 U |